Hepatic congestion is the main pathological sign in concanavalin A-induced acute hepatitis in Bama mini-swine
نویسندگان
چکیده
Objective: The plant lectin concanavalin A (ConA) induced acute hepatitis in mice is a commonly used animal model applied in acute immune-mediated hepatitis studies, but it is not suitable for large animal model of hepatic injury to promote the study of bio-artificial liver support systems in humans and help to assist pre-clinical evaluation. During producing Bama mini-swine model of hepatic injury, we find Hepatic congestion is dispensable for this model. Methods: Seven Obama mini-swines in the model group were exposed to ConA by intravenous injection (5 mg/kg) into the vein of auricular back. The other 3 miniswines in the control group was administered with equal volume of saline. Blood was collected from precaval vein at 0 h, 6 h and 24 h after injection. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum creatinine (SCr) were determined. Portal vein pressure was measured. Animals surviving 24 h were euthanased. Afterwards, Liver tissues were collected and liver/weight index was calculated. The histological analyses of liver, kidney and spleen were performed. Results: Our data show that hepatic congestion is the main pathological sign in concanavalin A-induced acute hepatitis model in mini-swine resulting in hepatitis congestion, intestinal bleeding, marked renal tubular necrosis and spleen. Conclusion: Hepatic congestion is the main pathological sign in concanavalin A-induced acute hepatitis in Bama mini-swine.
منابع مشابه
The Bama miniature swine is susceptible to experimental HEV infection
The hepatitis E virus (HEV) is one of the main causes of enterically transmitted hepatitis worldwide. Although the mortality rates associated with HEV are generally low, they can be up to 28% in HEV-infected pregnant women, and the elderly are more susceptible. The reasons for this selective severity are unclear, partially because there is no suitable, easy-to-use model in which to study HEV in...
متن کاملPathological Role of Interleukin-17 in Poly I:C-Induced Hepatitis
Immune-mediated responses were the main causes of liver damage during viral hepatitis, and recently viral RNA mimetic Poly I:C was used to induce a NK cell-dominated acute hepatitis. Interleukin-17A (IL-17A), the cytokine tightly associated with various autoimmune diseases, was known to play protective or pathological roles in LPS and ConA-induced hepatitis. However, its role in NK cell-mediate...
متن کاملProtective effect of interleukin-36 receptor antagonist on liver injury induced by concanavalin A in mice
Objective(s): Interleukin-36 receptor antagonist (IL-36Ra) is a new member of the IL-1 family that exhibits anti-inflammatory activity in a variety of inflammatory and immune diseases. Our purpose was to determine the effect of IL-36Ra on liver injury in a mouse hepatitis model induced by concanavalin A (ConA). Materials and Methods: Mic...
متن کاملOrthotopic Liver Transplantation for Etanercept-Induced Acute Hepatic Failure; a case report
The occurrence of hepatotoxicity following etanercept (tumor necrosis factor-alpha antagonist) prescription, has been studied well. However, an acute hepatic failure leading to liver transplant as an adverse effect of this drug has not been reported in the literature. In this article, we are going to present a case of acute liver failure followed by liver transplantation, in a 32 years old man ...
متن کاملEvaluation of the role of TLR4 in endotoxin-induced hepatic encephalopathy in rats with biliary cirrhosis
Background: Hepatic encephalopathy is defined as a neuropsychiatric brain dysfunction in acute or chronic liver failure. Infection and inflammation have crucial role in its pathophysiology. The purpose of our study was to demonstrate the relationship between toll-like receptor 4 (TLR4) expression and the encephalopathy induced with endotoxin in biliary cirrhotic rats. Methods: The present stud...
متن کامل